Coding

Part:BBa_K4348001:Design

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-08-22)


ismA_his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was codon optimized for expression in E. coli


Source

Genomic sequence from Eubacterium coprostanoligenes

References